spoIIAB

spoIIAB
168

anti-sigma factor to SigF, protein serine kinase, phosphorylates SpoIIAA

Locus
BSU_23460
Molecular weight
16.21 kDa
Isoelectric point
4.38
Protein length
146 aaSequenceBlast
Gene length
441 bpSequenceBlast
Function
control of SigF activity; phosphorylation and inactivation of SpoIIAA
Product
anti-sigma factor, protein serine kinase
Essential
no
Synonyms
spoIIAB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2172 (Galperin et al., 2021)

Gene
Coordinates
2,444,208 → 2,444,648
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology PubMed
The protein
Catalyzed reaction/ biological activity
phosphorylation of SpoIIAA
negative regulator (by binding) of SigF
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Protein family
anti-sigma-factor family (with RsbW, according to UniProt)
Structure
1L0O (PDB) (complex with SigF, Geobacillus stearothermophilus) PubMed
1TIL (PDB) (complex with SpoIIAA, Geobacillus stearothermophilus) PubMed
P10728 (AlphaFold)
Expression and Regulation
Operons
Genes
spoIIAA-spoIIAB-sigF
Description
PubMed
Regulation
''spoIIAA'': expressed early during sporulation
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
AbrB: repression, PubMed, in abrB regulon
SinR: repression, in sinR regulon
Sigma factors
SigF: sigma factor, in sigF regulon
SigG: sigma factor, in sigG regulon
SigH: sigma factor, in sigH regulon
Open in new tab

spoIIAA→sigF

2025-03-15 00:06:01

ghost

135

64b4f19f43ec57e308620c649e41be0eaadae6be

781B7F1839769E51F61804AF186C6D2AF7A0DA95

Genes
dacF-spoIIAA-spoIIAB-sigF
Description
PubMed
Regulation
''spoIIAA'': expressed early during sporulation
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigF: sigma factor, PubMed, in sigF regulon
SigG: sigma factor, in sigG regulon
Open in new tab

dacF→sigF

2025-03-09 18:32:40

ghost

138

3937ee398a65a809f372c4c59231b1d2e3c32629

AEBA18A2591E8C2953E0D2D62B0F6CD480E5CB2B

Biological materials
Mutant
BKE23460 (ΔspoIIAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACTCAAGGTGCATTTCAT,  downstream forward: _UP4_CTTTGTAATTAAGGAGATTT
BKK23460 (ΔspoIIAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACTCAAGGTGCATTTCAT,  downstream forward: _UP4_CTTTGTAATTAAGGAGATTT
Labs working on this gene/protein
Charles Moran, Emory University, NC, USA homepage
References
Reviews
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Original Publications
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C8B867758330355F44C63CC284DC7AA3061FB9F1

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Time of last update: 2025-03-15 13:51:30

Author of last update: Jstuelk