folD

folD
168

methylenetetrahydrofolate dehydrogenase (NADP)

Locus
BSU_24310
Molecular weight
30.53 kDa
Isoelectric point
5.6
Protein length
283 aaSequenceBlast
Gene length
852 bpSequenceBlast
Function
formylation of Met-tRNA(fMet)
Product
methylenetetrahydrofolate dehydrogenase (NADP)
Essential
no
E.C.
1.5.1.5
Synonyms
folD, yqiA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0190 (Galperin et al., 2021)

This gene is a member of the following regulons

Stringent response, PurR regulon

Gene
Coordinates
2,528,404  2,529,255
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
inactivation of ''folD suppresses the synthetic lethality of the defA defB'' double mutant PubMed
The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP+ --> 5,10-methenyltetrahydrofolate + NADPH (according to UniProt)
5,10-methenyltetrahydrofolate + H2O --> (6S)-10-formyltetrahydrofolate + H+ (according to UniProt)
Protein family
tetrahydrofolate dehydrogenase/cyclohydrolase family (single member, according to UniProt)
Structure
1B0A (PDB) (from E. coli, 52% identity) PubMed
P54382 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
nusB-folD
Description
PubMed
Regulation
repressed in the presence of purine nucleotides (PurR) PubMed
Regulatory mechanism
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
stringent response: negative regulation, in stringent response
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nusB→folD

2025-03-09 07:06:48

ghost

88

c4252d054332d8a4aedfc8005fc5ac765b3f1223

9765ECDFA2520F690845D25E096C6F679262B232

Biological materials
Mutant
BKE24310 (folD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA,  downstream forward: _UP4_TAGATAATTGGAGATATTCA
BKK24310 (folD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA,  downstream forward: _UP4_TAGATAATTGGAGATATTCA
References
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71FC6E75694B7066D808BAD736140569875E5E5C

Page visits: 3583

Time of last update: 2025-03-13 05:00:27

Author of last update: Jstuelk