defB

defB
168

N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine

Locus
BSU_14560
Molecular weight
20.51 kDa
Isoelectric point
5.46
Protein length
184 aaSequenceBlast
Gene length
555 bpSequenceBlast
Function
utilization of S-methyl-cysteine
Product
N-formylcysteine deformylase
Essential
no
E.C.
3.5.1.88
Synonyms
defB, ykrB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0242 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,526,195  1,526,749
Phenotypes of a mutant
a ''defA defB double mutant is not viable unless the cells acquire suppressor mutations in fmt, glyA or folD'' PubMed
no growth with S-methyl cysteine PubMed
inactivation of ''defB'' reduces sporulation efficiency to 9% that of wild type cells; engulfment and fission defects, with reduced SigG activity PubMed
The protein
Catalyzed reaction/ biological activity
N-acetylcysteine - cysteine + acetate PubMed
H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
Protein family
polypeptide deformylase family (with DefA, according to UniProt)
Structure
1LQY (PDB) (complex with antibiotic actinonin, Geobacillus stearothermophilus, 67% identity) PubMed
Q45495 (AlphaFold)
Paralogous protein(s)
DefA
Expression and Regulation
Operons
Genes
defB
Description
PubMed
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defB

2025-03-08 22:58:38

Jstuelk

59

25e4ac254d10db0959d4e720b04c93c280201f3f

79FAAC433B03035AAA5B10BAFB040F6C3DBA55F9

Biological materials
Mutant
MGNA-A901 (ykrB::erm), available at the NBRP B. subtilis, Japan
BKE14560 (defB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA,  downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC
BKK14560 (defB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA,  downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC
References
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53B760316B19E142FC3ECAD2CFAA540377B08BBC

Page visits: 4650

Time of last update: 2025-03-12 17:23:00

Author of last update: Melvin.boenninger