dhbB

dhbB
168

isochorismatase

Locus
BSU_31970
Molecular weight
34.95 kDa
Isoelectric point
4.43
Protein length
312 aaSequenceBlast
Gene length
939 bpSequenceBlast
Function
biosynthesis of the siderophore bacillibactin
Product
isochorismatase
Essential
no
E.C.
3.3.2.1
Synonyms
dhbB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3433 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,287,675  3,288,613
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium PubMed
The protein
Catalyzed reaction/ biological activity
Isochorismate + H2O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)
Protein family
Isochorismatase family (according to UniProt)
Domains
Carrier domain (aa 229-302) (according to UniProt)
Structure
2FQ1 (PDB) (from E. coli, 49% identity) PubMed
P45743 (AlphaFold)
Expression and Regulation
Operons
Genes
dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ
Description
PubMed
Regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
expression is increased in the presence of pulchimerrin PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
AbrB: repression, PubMed, in abrB regulon
Kre: repression, in kre regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon
Additional information
the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed
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dhbA→ybdZ

2025-03-12 23:03:40

Jstuelk

146

875CBC1C026244834E2EFBC1140499985C0AB89D

CCA159D50C58E2078C51F543EDA686D77F6612EE

Biological materials
Mutant
BKE31970 (dhbB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAACACTTCCTCTCA,  downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
BKK31970 (dhbB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAACACTTCCTCTCA,  downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
References
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1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA

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Time of last update: 2025-03-12 12:57:04

Author of last update: Jstuelk