cotE

cotE
168

outer spore coat morphogenetic protein, controls the assembly of the outer spore coat layer

Locus
BSU_17030
Molecular weight
20.83 kDa
Isoelectric point
4.14
Protein length
181 aaSequenceBlast
Gene length
546 bpSequenceBlast
Function
assembly of the outer spore coat
Product
spore coat morphogenetic protein
Essential
no
Synonyms
cotE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5880 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,775,067  1,775,612
Phenotypes of a mutant
mis-assembly of the outer spore coat PubMed
inactivation of ''cotE'' reduces sporulation efficiency to 49.1% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
assembly of the CotC-CotU complex PubMed
Protein family
cotE family (single member, according to UniProt)
Structure
P14016 (AlphaFold)
Localization
encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore  PubMed
outer spore coat, localization depends on SpoIVA PubMed
Expression and Regulation
Operons
Genes
cotE
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed
Regulatory mechanism
GerE: repression, PubMed, in gerE regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
SigK: sigma factor, PubMed, in sigK regulon
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cotE

2025-03-08 17:55:30

ghost

68

df5e14a1265e3e2e508fd6b0e9f2d3f90863fdc2

02BCF0CE206BD81EBBAD019076E714D6C44C8C64

Biological materials
Mutant
1S105 ( cotE::cat), PubMed, available at BGSC
BKE17030 (cotE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG,  downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
BKK17030 (cotE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG,  downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Adriano Henriques, Lisbon, Portugal homepage
References
Reviews
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Original Publications
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Time of last update: 2025-03-16 12:33:46

Author of last update: Jstuelk