zagA

zagA
168

zinc metallochaperone, ZTP activated GTPase A

Locus
BSU_03360
Molecular weight
45.15 kDa
Isoelectric point
4.4
Protein length
397 aaSequenceBlast
Gene length
1194 bpSequenceBlast
Function
supply of zinc from RpmE to FolE
Product
ZTP activated GTPase A
Essential
no
Synonyms
zagA, yciC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0523 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
366,063  367,256
The protein
Catalyzed reaction/ biological activity
has GTPase and ZTPase activity PubMed
mobilization of zinc from RpmE (after insertion of RpmEB into the ribosome), to provide it to FolE PubMed
the interaction with FolE facilitates the supply of zinc to FolE under conditions of zinc limitation PubMed
Protein family
SIMIBI class G3E GTPase family (single member, according to UniProt)
Domains
CobW C-terminal domain (aa 259-374) (according to UniProt)
Structure
1NIJ (PDB) (YjiA from E. coli, 27% identity) PubMed
P94400 (AlphaFold)
Modification
phosphorylated on Arg-58 PubMed
Effectors of protein activity
ZTP (stimulates interaction with FolE) reference
Kinetic information
KM for GTP: 40 µM PubMed
KM for ZTP: 36 µM PubMed
Localization
membrane associated PubMed
Expression and Regulation
Operons
Genes
yczL-zagA
Description
PubMed
Regulation
''folE2'': induced by zinc starvation (Zur) PubMed
Regulatory mechanism
Zur: repression, in the presence of zinc PubMed, in zur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

yczL→zagA

2025-03-11 00:28:44

ghost

82

f1a9da8a2145cd33659ba3d54e0d9a81c7389ae4

11D924483BF509F89BDCD36CAA9727DC5156792B

Genes
folEB-yciB-yczL-zagA
Description
PubMed
Regulation
folE2: induced by zinc starvation (Zur) PubMed
Regulatory mechanism
Zur: repression, in the presence of zinc PubMed, in zur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

folEB→zagA

2025-03-10 19:32:48

Jstuelk

124

ad7d4075bafccef586e192b5f9b59ca4750bd357

154D780A688DFF455944F618E9A71FDF4E6BA39E

Biological materials
Mutant
MGNA-B999 (yciC::erm), available at the NBRP B. subtilis, Japan
BKE03360 (zagA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAGTGCCTCCATTAA,  downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT
BKK03360 (zagA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAGTGCCTCCATTAA,  downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
References
Reviews
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Original Publications
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75DB07A2704FEC2932BD0CDD24E2B3454E9551E1

Page visits: 4020

Time of last update: 2025-03-14 22:40:14

Author of last update: Melvin.boenninger