leuC

leuC
168

3-isopropylmalate dehydratase (large subunit)

Locus
BSU_28260
Molecular weight
52.23 kDa
Isoelectric point
6.13
Protein length
472 aaSequenceBlast
Gene length
1419 bpSequenceBlast
Function
biosynthesis of leucine
Product
3-isopropylmalate dehydratase (large subunit)
Essential
no
E.C.
4.2.1.33
Synonyms
leuC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0065 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,889,552 2,890,970
The protein
Catalyzed reaction/ biological activity
(2R,3S)-3-isopropylmalate --> (2S)-2-isopropylmalate (according to UniProt)
Protein family
aconitase/IPM isomerase family (with CitB, according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
4KP2 (PDB) (from Methanococcus jannaschii, 35% identity)
P80858 (AlphaFold)
Modification
phosphorylated on Arg-81 PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
Description
PubMed
Regulation
expression is stimulated in the presence of glucose (CcpA) PubMed
Regulatory mechanism
T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
TnrA: repression, PubMed, in tnrA regulon
CodY: repression, PubMed PubMed, in codY regulon
CcpA: activation, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for leuA PubMed
the T-box RNA is degraded by RNase Y PubMed
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ilvB→leuD

2025-03-01 21:15:25

Jstuelk

144

905a0fc4b9e1945f65cf0820ea8bda45e4ac716c

931C13BE07545B8037A118810CF4C8E29210A7CE

Other regulations
FsrA: translation repression, PubMed
Biological materials
Mutant
BKE28260 (leuC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG
BKK28260 (leuC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG
References
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Page visits: 7054

Time of last update: 2025-03-12 15:25:38

Author of last update: Melvin.boenninger