Difference between revisions of "GltX"
| Line 61: | Line 61: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00920&redirect=T BSU00920] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltX-cysES-yazC-yacOP.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltX-cysES-yazC-yacOP.html] | ||
| Line 96: | Line 97: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00920&redirect=T BSU00920] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3AKZ 3AKZ] (complex with tRNA-Gln from ''Thermotoga maritima'', 38% identity, 62% similarity) {{PubMed|20882017}} | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3AKZ 3AKZ] (complex with tRNA-Gln from ''Thermotoga maritima'', 38% identity, 62% similarity) {{PubMed|20882017}} | ||
Revision as of 12:48, 2 April 2014
- Description: glutamyl-tRNA synthetase, universally conserved protein
| Gene name | gltX |
| Synonyms | |
| Essential | yes PubMed |
| Product | glutamyl-tRNA synthetase |
| Function | translation |
| Gene expression levels in SubtiExpress: gltX | |
| Interactions involving this protein in SubtInteract: GltX | |
| Metabolic function and regulation of this protein in SubtiPathways: gltX | |
| MW, pI | 55 kDa, 5.021 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | ispF, cysE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00920
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU00920
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU00920
- Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed
- UniProt: P22250
- KEGG entry: [3]
- E.C. number: 6.1.1.17
Additional information
Expression and regulation
- Regulation:
- expression transiently increases in the forespore PubMed
- Regulatory mechanism:
- Additional information:
- the mRNA is very stable (half-life > 15 min) PubMed
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
