rqcP

rqcP
168

ribosome-associated quality control factor, stabilizes the P-site tRNA conformation, similar to heat shock protein Hsp15, processivity factor essential for poly-alanine synthesis during ribosome quality control elongation by RqcH

Locus
BSU_00590
Molecular weight
9.58 kDa
Isoelectric point
10.14
Protein length
86 aaSequenceBlast
Gene length
261 bpSequenceBlast
Function
ribosome-associated quality control
Product
ribosome-associated quality control factor, P-site tRNA stabilizing factor
Essential
no
Synonyms
yabO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1188 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
67,877 → 68,137
The protein
Catalyzed reaction/ biological activity
translocates the tRNA moiety from the A site to the P site of stalled 50S ribosomal subunits PubMed
required for the synthesis of the poly-Ala degron tail to resolve stalled ribosomes PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
Domains
S4 RNA-binding domain (aa 1-62) (according to UniProt)
Structure
7AQC (PDB) PubMed
3BBU (PDB) (from E. coli, 36% identity) PubMed
P37557 (AlphaFold)
Expression and Regulation
Operons
Genes
yabM-yabN-rqcP-yabP-shfA-divIC-yabR
Description
PubMed
Regulation
expressed during sporulation (SigE) PubMed
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigE: sigma factor, PubMed, in sigE regulon
SigW: sigma factor, PubMed, in sigW regulon
SigD: sigma factor, PubMed, in sigD regulon
SigX: sigma factor, PubMed, in sigX regulon
Open in new tab

yabM→yabR

2025-03-14 21:01:33

Jstuelk

116

93952be38722510f984794e138450cd80a9f7d48

0CF3909333400A3BFE18E32016883124FFC3D75B

Biological materials
Mutant
MGNA-B916 (yabO::erm), available at the NBRP B. subtilis, Japan
BKE00590 (ΔrqcP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAATTTATCTAATCTCA,  downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
BKK00590 (ΔrqcP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAATTTATCTAATCTCA,  downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
References
Reviews
Loading
Original Publications
Loading

B0CB19A3E3E84E12BF42BB050394C8A059A89359

Page visits: 3756

Time of last update: 2025-03-16 08:22:53

Author of last update: Jstuelk