sigW

sigW
168

RNA polymerase ECF-type sigma factor SigW, required for the adaptation to membrane active agents, activated by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100

Locus
BSU_01730
Molecular weight
21.57 kDa
Isoelectric point
8.61
Protein length
187 aaSequenceBlast
Gene length
564 bpSequenceBlast
Function
adaptation to membrane-active compounds
Product
RNA polymerase ECF-type sigma factor SigW
Essential
no
Synonyms
sigW, ybbL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
194,849  195,412
Phenotypes of a mutant
sensitive to -lactam antibiotics such as cefuroxime and to fosfomycin PubMed
Inactivation of ''alsR, sigD and sigW increases competitive fitness of Bacillus subtilis'' under non-sporulating conditions PubMed
The protein
Protein family
Sigma-70 factor family (according to UniProt)
ECF subfamily (according to UniProt)
Structure
5WUQ (PDB) (complex with the cytoplasmic domain of RsiW) PubMed
Q45585 (AlphaFold)
Effectors of protein activity
RsiW acts as antagonist of SigW (anti-SigW)
Expression and Regulation
Operons
Genes
sigW-rsiW
Description
PubMed
Regulation
induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigW: sigma factor, PubMed, in sigW regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
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sigW→rsiW

2025-03-17 13:17:34

ghost

149

f173494f184da3acb9643a06d22253706f42bb28

A73BFA90A2F503CCE374A383B36C83668CF7F3DD

Biological materials
Mutant
1A905 ( sigW::erm), PubMed, available at BGSC
BKE01730 (sigW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTATCTAACCTCTGC,  downstream forward: _UP4_AGGGATCTTTAAGTGGGGTG
BKK01730 (sigW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTATCTAACCTCTGC,  downstream forward: _UP4_AGGGATCTTTAAGTGGGGTG
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
Thomas Wiegert, University of Bayreuth, Germany Wiegert-Dateien/Thomas Wiegert.html Homepage
References
Reviews
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Other original publications
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The SigW regulon
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3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D

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Time of last update: 2025-03-17 20:58:59

Author of last update: Jstuelk