ykfB

ykfB
168

L-Ala-D/L-Glu epimerase

Locus
BSU_12980
Molecular weight
39.31 kDa
Isoelectric point
5.91
Protein length
366 aaSequenceBlast
Gene length
1101 bpSequenceBlast
Function
cell wall metabolism
Product
L-Ala-D/L-Glu epimerase
Essential
no
E.C.
null
Synonyms
ykfB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4948 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,366,844  1,367,944
The protein
Catalyzed reaction/ biological activity
L-alanyl-L-glutamate --> L-alanyl-D-glutamate (according to UniProt)
Protein family
mandelate racemase/muconate lactonizing enzyme family (according to UniProt)
Structure
1TKK (PDB) (complex with substrate),  1JPM (PDB)
O34508 (AlphaFold)
Expression and Regulation
Operons
Genes
dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD
Description
PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
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dppA->ykfD

2025-03-15 07:41:12

Bzhu

138

9978370314fc05087eca13c4f3ce94e92ce93ff2

E2A0CC9BE866DBD9F0E3A5F48EE05465C14EFD9D

Biological materials
Mutant
MGNA-A741 (ykfB::erm), available at the NBRP B. subtilis, Japan
BKE12980 (ykfB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA,  downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA
BKK12980 (ykfB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA,  downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA
References
Reviews
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Original Publications
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77EFC47449D8CC17651ED61C41AC605FCEF01270

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Time of last update: 2025-03-16 09:49:54

Author of last update: Jstuelk