yhcT
168
pseudouridylate synthase, involved in pseudouridylation of 23S rRNA positions 1940, 1944, and 1946
locus
BSU_09210
Molecular weight
33.59 kDa
pI
9.64
function
23S rRNA modification
product
putative [wiki|sporulation]-specific pseudouridylate synthase
essential
no
synonyms
yhcT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
995,624 996,532
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The protein
Catalyzed reaction/ biological activity
uridine in RNA --> ψ-uridine in 23S rRNA at positions 1940, 1944, and 1946 [pubmed|32629984]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO] and [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB], according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 19-90) (according to UniProt)
Structure
[PDB|1V9F] (RluD from E. coli, 36% identical) [pubmed|15078091]
[AF|P54604]
Paralogous protein(s)
[protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO], [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]
Expression and Regulation
Operons
genes
[gene|964F04481DFA789CB0F7387B066BE2E2CF88D8E5|S319]-[gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A666 (yhcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/666 NBRP B. subtilis, Japan]
BKE09210 ([gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT, downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC
BKK09210 ([gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT, downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC
References
Research papers
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Time of last update: 2024-12-01 22:52:27
Author of last update: Jstuelk