whiA
168
similar to transcription repressor of the [gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] operon, involved in Z ring assembly, required for normal chromosome segregation
locus
BSU_34750
Molecular weight
36.18 kDa
pI
9.48
function
control of Z ring asembly and chromosome segregation
product
[category|SW.1.1.8|Cell division] protein
essential
no
synonyms
whiA, yvcL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1481 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,569,573 3,570,523
Phenotypes of a mutant
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) [Pubmed|24097947]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [gene|search|whiA ][gene|search|parB ]double mutant is not viable, this can be suppressed by inactivation of [gene|search|yneA ][pubmed|29378890]
a [gene|search|whiA ][gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] double mutant is not viable [pubmed|29378890]
the defects of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant can be suppressed by inactivation of either ''[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]'', ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'', or ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' [Pubmed|24097947]
highly sensitive for DNA damaging agents [pubmed|29378890]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
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The protein
Catalyzed reaction/ biological activity
in Enterococcus faecalis: transcription repressor of the [gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] operon [pubmed|38512984]
Protein family
WhiA family (single member, according to UniProt) [Pubmed|10986251]
[wiki|Domains]
suggested HTH domain at the N terminus [Pubmed|19836336]
Structure
[PDB|3HYI] (Crystal structure of full-length DUF199/WhiA from ''Thermotoga maritima'')
[PDB|3HYJ] (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
[AF|O06975]
[wiki|Localization]
localizes to the nucleoid [Pubmed|24097947]
Expression and Regulation
Operons
genes
[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
description
[Pubmed|9237995]
regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) ([protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]) [pubmed|38512984]
regulatory mechanism
[protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]: transcription repression, described for Enterococcus faecalis [pubmed|38512984], in [regulon|protein:1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16272399], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]' [PubMed|20525796]
Biological materials
Mutant
MGNA-B639 (yvcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1638 NBRP B. subtilis, Japan]
GP231 (''amyE::P[gene|751D0B909922D4D931FF3B2285CF142D966FCD48|rpsJ]-lacZ, [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]::cat'') available in [wiki| Jörg Stülke]'s lab; plasmid pGP1468 was used to disrupt the gene.
BKE34750 (Δ[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
BKK34750 (Δ[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
Expression vectors
pGP1469 for expression in ''B. subtilis'' (in [wiki|pGP382], with C-terminal STREP-TAG), available in [wiki| Jörg Stülke]'s lab
pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki| Jörg Stülke]'s and [wiki|Boris Görke]'s labs
FLAG-tag construct
GP782 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Leendert Hamoen]'s lab [Pubmed|24097947]
References
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Time of last update: 2024-11-29 20:45:09
Author of last update: Jstuelk