kinD
168
osmo-sensing two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F], part of the [wiki|phosphorelay], checkpoint protein that links [wiki|sporulation] initiation to [wiki|biofilm formation]
locus
BSU_13660
Molecular weight
56.54 kDa
pI
6.74
function
initiation of [wiki|sporulation]
product
two-component sensor kinase
essential
no
synonyms
kinD, ykvD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5808 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,431,486 1,433,006
Phenotypes of a mutant
deletion of ''kinD'' suppresses the [wiki|sporulation] defect of matrix mutants, while its overproduction delays [wiki|sporulation] [Pubmed|20689749]
inactivation of ''[gene|511E71BB1981758857854C8E9BF657287CE60C11|kinD]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
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The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F], regulates the onset of sporulation by inhibiting the activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] until matrix, or a component therein, is sensed [http://mbio.asm.org/content/1/1/e00035-10.full PubMed]
dual role as a [wiki|phosphatase] or a [wiki|kinase], activity is linked to the presence of extracellular matrix in the biofilms [Pubmed|20689749]
mainly active in the younger, outer regions of a colony (with [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC]) [Pubmed|21097618]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments, between them the extracellular pyruvate-binding sensing domain that consists of tandem PAS-like domains [Pubmed|23436677,22716461]
see [Pubmed|22716461] for a scheme of the domain organization
[wiki|Histidine kinase domain] (aa 298-505) (according to UniProt)
Structure
[PDB|4DBJ] (extracytoplasmic sensing domain) [Pubmed|23436677]
[AF|O31671]
Modification
autophosphorylation on a His residue
Effectors of protein activity
activity is stimulated by direct or indirect interaction with [protein|4DAB7847EA3848A34432C80124464543FA3DCA0F|med] [Pubmed|21622736]
may respond to the presence of extracellular glycerol [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]
L-malate seems to trigger KinD activity [Pubmed|22716461], but this effect may be indirect due to the excretion of pyruvate that directly binds the extracytoplasmic sensing domain of KinD [Pubmed|23436677]
kinase activity is triggered and phosphatase activity is decreased by increased osmotic pressure [Pubmed|22882172]
activity is triggered in the presence of glycerol + manganese [Pubmed|23564171]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|511E71BB1981758857854C8E9BF657287CE60C11|kinD]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B323 (ykvD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1322 NBRP B. subtilis, Japan]
BKE13660 ([gene|511E71BB1981758857854C8E9BF657287CE60C11|kinD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACCTGATTAGAGGTA, downstream forward: _UP4_TAGCCCCCCTGACCATGTCA
BKK13660 ([gene|511E71BB1981758857854C8E9BF657287CE60C11|kinD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACCTGATTAGAGGTA, downstream forward: _UP4_TAGCCCCCCTGACCATGTCA
References
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Time of last update: 2024-11-29 20:45:29
Author of last update: Jstuelk