dnaA
168
AAA ATPase, [category|SW.3.1.1|replication] initiation protein
locus
BSU_00010
Molecular weight
50.70 kDa
pI
6.03
function
[category|SW.3.1.1|DNA replication]
product
replication initiation protein
essential
yes
synonyms
dnaA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0593 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
410 → 1,750
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC]
binds to a pair of double-stranded DNA binding sites ([protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]‐boxes) that guide the protein onto adjacent ssDNA binding motifs ([protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]‐trios) where the protein assembles into an oligomer that stretches DNA to promote origin unwinding [pubmed|34197592]
Protein family
DnaA family (single member, according to UniProt)
[wiki|Domains]
Domain I: aa 1 ... 80: oligomerization and interaction with [protein|2AEF6D56D884CA6A0BD50FABD27F405DC2B506C7|dnaB] [pubmed|10572294]
Domain II: aa 81 ... 114: flexible loop [pubmed|10572294]
Domain III: aa 115 ... 353: AAA+ motif, binding of ATP, ATPase activity, second site for interaction with [protein|2AEF6D56D884CA6A0BD50FABD27F405DC2B506C7|dnaB], local DNA unwinding [pubmed|10572294]
Domain IV: HTH motif for specific dsDNA binding ([protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] boxes) [pubmed|10572294]
Structure
[PDB|2Z4R] (from ''Thermotoga maritima '') [Pubmed|18216012]
[AF|P05648]
Effectors of protein activity
[protein|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA] displaces [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] from the replication origin [Pubmed|19682252]
[protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] inhibits co-operative binding of DnaA to the ''oriC'' DNA [Pubmed|21895792]
DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA], [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] or [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] with the AAA domain of DnaA [Pubmed|23909787,22286949]
interaction with [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD] inhibits the ability of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] to cooperatively bind to DNA [Pubmed|22821970]
DNA gyrase ([protein|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]-[protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]) inhibits association of DnaA with oriC [pubmed|29396913]
[wiki|Localization]
throughout the cytoplasm [Pubmed|10844689]
co-localizes with the origin region and replication forks throughout the cell cycle [pubmed|28166228]
Expression and Regulation
Operons
genes
[gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
description
[Pubmed|2987848]
regulation
negatively controlled by [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|2168872] and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2987848], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
[wiki|Alan Grossman], MIT, Cambridge, MA, USA
[wiki|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]
References
Reviews
Original Publications
The [wiki|DnaA regulon]
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Time of last update: 2024-11-28 19:38:28
Author of last update: Jstuelk