greA
168
transcription elongation factor, resolves stalled [wiki|RNA polymerase] at promoter or promoter-proximal regions
locus
BSU_27320
Molecular weight
17.00 kDa
pI
4
function
transcription elongation
product
transcription elongation factor
essential
no
synonyms
greA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0782 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,791,494 2,791,967
Phenotypes of a mutant
[wiki|RNA polymerase] accumulates in promoter-proximal regions of many genes [Pubmed|21515770]
loss of [wiki|genetic competence] [pubmed|33897624,28189581]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
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The protein
Catalyzed reaction/ biological activity
resolves stalled [wiki|RNA polymerase] at promoter or promoter-proximal regions
required for repair of DNA lesions during growth [pubmed|32041798]
promotes error-prone DNA repair during stress to promote genetic diversity [pubmed|32041798]
Protein family
GreA/GreB family (single member, according to UniProt)
Structure
[PDB|1GRJ] (from ''E. coli'', 43% identity) [Pubmed|7854424]
[AF|P80240]
[wiki|Localization]
uniformly distributed throughout the nucleoid [Pubmed|16707701,21515770]
Expression and Regulation
Operons
genes
[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
description
[Pubmed|22383849]
genes
[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
description
[Pubmed|22383849]
Biological materials
Mutant
BP351 ([gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]::''cat'') available in [wiki|Fabian Commichau]'s lab
GP2612 (Δ[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]::aphA3) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
BKE27320 (''[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]''::''ermC'') (available in the BGSC and in [wiki|Jörg Stülke]'s lab)
BKE27320 ([gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
BKK27320 ([gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCCTTCACTCCTTCT, downstream forward: _UP4_TAATATCTGTTTAGCAGCGA
Expression vectors
C-terminal Strep-tag, for [wiki|SPINE], expression in ''B. subtilis'', in [wiki|pGP382]: pGP2547, available in [wiki|Jörg Stülke]'s lab [pubmed|28189581]
FLAG-tag construct
GP2525 (in [wiki|pBP43]), expression of '' greA-FLAG''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP2546 in ([wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
GP2537 (kan), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP2527 (in [wiki|pBP43]), expression of '' greA-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:45:40
Author of last update: Jstuelk