mutL
168
sequence-unspecific endonuclease, DNA mismatch repair
locus
BSU_17050
Molecular weight
70.25 kDa
pI
5.58
function
DNA repair
product
DNA mismatch repair protein, sequence-unspecific endonuclease
essential
no
synonyms
mutL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0323 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,778,337 1,780,220
Phenotypes of a mutant
60-fold increased mutation rate [Pubmed|23882084]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]
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The protein
Protein family
DNA mismatch repair mutL/hexB family (single member, according to UniProt)
Structure
[PDB|3KDK] (C-terminal domain) [Pubmed|20603082]
[PDB|6E8D] (complexed with [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]) [Pubmed|30916336]
[AF|P49850]
[wiki|Localization]
forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]
Expression and Regulation
Operons
genes
[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]
description
[Pubmed|8760914]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|mutL]' and '[protein|search|ymzD]' [PubMed|20525796]
Biological materials
Mutant
GP1190 ([gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [Pubmed|22178973]
1A833 ( ''mutL''::''spec''), [Pubmed|11779496], available at [http://bgsc.org/search.php?Search=1A833 Bacillus Genetic Stock Center]
BKE17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC, downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
BKK17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC, downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
References
Reviews
Original Publications
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Time of last update: 2024-12-01 23:31:18
Author of last update: Jstuelk