mfd
168
[wiki|transcription]-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation, required for increased mutagenesis of lagging strand genes
locus
BSU_00550
Molecular weight
133.59 kDa
pI
5.37
function
promotes strand-specific DNA repair by displacing
product
[wiki|transcription]-repair coupling factor
essential
no
ec
3.6.4.-
synonyms
mfd
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1197 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
60,430 63,963
Phenotypes of a mutant
in an ''mfd'' knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed [http://www.pubmed.com/16950921 PubMed]
strongly reduced stationary phase mutagenesis [Pubmed|27399782]
reduced formation of [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB] or [gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE] suppressor mutants after mitomycin treatment [pubmed|34339280]
increased UV-induced mutagenesis via [protein|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]/ [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]-mediated translesion synthesis [Pubmed|24118570]
the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants [Pubmed|24296669]
sensitive to blue light-induced DNA damage [pubmed|30054368]
sensitive to oxidative stress [pubmed|30691388]
suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactivation [pubmed|32793628]
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The protein
Catalyzed reaction/ biological activity
promotes strand-specific DNA repair by displacing [wiki|RNA polymerase] stalled at a nucleotide lesion and directing the (A)BC excinuclease to RNA damage site
is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA] [Pubmed|9535092] and [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|21699902])
required for the processing of genetic damage during [wiki|sporulation] [Pubmed|24118570]
required for repair of DNA lesions during growth [pubmed|32041798]
promotes error-prone DNA repair during stress to promote genetic diversity [pubmed|32041798]
couples transcription to nucleotide excision repair (NER) to eliminate DNA distorting lesions during sporulation [pubmed|33510358]
Protein family
N-terminal part: UvrB family (with [protein|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB], according to UniProt)
C-terminal part: [wiki|helicase family] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 638-799) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 820-974) (according to UniProt)
Structure
[PDB|2EYQ] (Mfd from ''E. coli'') [Pubmed|16469698]
[PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] beta1 domain) [Pubmed|20702425]
[AF|P37474]
Paralogous protein(s)
[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU], [protein|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]-[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]-[gene|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]
description
[pubmed|22383849]
expression
expression is heterogeneous [pubmed|35171018]
Biological materials
Mutant
GP3519 (Δ[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::''kan''), available in [wiki|Jörg Stülke]'s lab
GP1167 (Δ[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::''ermC''), available in [wiki|Jörg Stülke]'s lab [Pubmed|22178973]
BKE00550 (''[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s labs) [pubmed|28189581]
BKE00550 ([gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGCCCCTCCTCTCTG, downstream forward: _UP4_TAAATTTTGTTACTCTCTGG
BKK00550 ([gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGCCCCTCCTCTCTG, downstream forward: _UP4_TAAATTTTGTTACTCTCTGG
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
GFP fusion
GP1510 (spc, based on [wiki|pGP1870], [wiki|pGP1389]-derivative ), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-01 23:31:23
Author of last update: Jstuelk