mutS
168
DNA mismatch repair (mismatch recognition protein)
locus
BSU_17040
Molecular weight
97.39 kDa
pI
5.19
function
DNA repair
product
mismatch recognition protein
essential
no
synonyms
mutS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0249 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,775,745 → 1,778,321
Phenotypes of a mutant
60-fold increased mutation rate [Pubmed|23882084]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]
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The protein
Catalyzed reaction/ biological activity
MutS recognizes mismatches in the DNA and recruits [protein|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL] to the site of mismatch recognition
assists DNA strand exchange by facilitating [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly, and indirectly re-engagement with the homologous 5'-end of the linear duplex [pubmed|30814990]
modulates bidirectional [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-mediated integration of divergent sequences [pubmed|30814990]
Protein family
DNA mismatch repair mutS family (with [protein|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU], according to UniProt)
Structure
[PDB|1NG9] (from ''E. coli'', 39% identity) [Pubmed|12554674]
[AF|P49849]
[wiki|Localization]
forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]
replication-active region of the DNA, via [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] [Pubmed|23228104]
MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the [wiki|replisome] region, even in the absence of appreciable mismatch formation [Pubmed|26575623]
Expression and Regulation
Operons
genes
[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]
description
[Pubmed|8760914]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|mutL]' and '[protein|search|ymzD]' [PubMed|20525796]
Biological materials
Mutant
GP1190 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [Pubmed|22178973]
1A833 ( ''mutS''::''spec''), [Pubmed|11779496], available at [http://bgsc.org/getdetail.php?bgscid=1A833 Bacillus Genetic Stock Center]
BKE17040 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTAATCCCTCACTA, downstream forward: _UP4_TTACATTAAAACGGGGTGAT
BKK17040 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTAATCCCTCACTA, downstream forward: _UP4_TTACATTAAAACGGGGTGAT
References
Reviews
Original Publications
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Time of last update: 2024-12-01 23:31:47
Author of last update: Jstuelk