parB

parB
168

CTP hydrolase, chromosome positioning near the pole and transport through the polar septum / antagonist of SMC complex to the origin of replication, coordination of early steps in DNA replication with establishment of a medial division site

locus
BSU_40960
Molecular weight
32.06 kDa
pI
8.42
Protein length
282 aaSequenceBlast
Gene length
849 bpSequenceBlast
function
chromosome positioning before asymmetric septation
product
[protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-loading protein
essential
no
synonyms
parB, spo0J, spo0JB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1475 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,205,556 → 4,206,404
Phenotypes of a mutant
a [gene|search|whiA ][gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] double mutant is not viable, this can be suppressed by inactivation of [gene|search|yneA ][pubmed|29378890]
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The protein
Catalyzed reaction/ biological activity
CTP + H2O —> CDP - H+ + phosphate [pubmed|37850647]
forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation [pubmed|29244022]
inhibits [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] dimerization and concomitant DNA-binding activity by stimulating [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] ATPase activity [Pubmed|21235642]
recruits the condensin complex to the DNA origin region [Pubmed|24440393]
the dimer binds specifcally to the centromere-like ''parS'' sequence [Pubmed|25572315]
prevents Z ring assembly over the bacterial nucleoid and helps fine tune the assembly of the Z ring at midcell during the cell cycle [pubmed|31152469]
Protein family
ParB family (with [protein|559DEDC9887B811EF80994526256ADC48BA51CE3|noc], according to UniProt)
[wiki|Domains]
N-terminal domain (NTD, aa 1 - 96): binds CTP and [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] [pubmed|34250901,10064607]
Central DNA-binding domain (CDBD, aa 102 - 216): binds parS DNA [pubmed|16306995]
C-terminal domain (CTD, aa 233 - 282): binding of non-specific DNA, dimerization, essential for DNA condensation [pubmed|29244022]
Structure
[PDB|4UMK] (complex with ''parS'' DNA)
[PDB|5U1G] [pubmed|28373206]
[PDB|5U1J] [pubmed|28373206]
[PDB|6SDK] (complex with CDP)
[AF|P26497]
Effectors of protein activity
binding of CTP stimulates the specific interaction with the [gene|search|parS] DNA [pubmed|34250901]
hydrolysis of CTP is essential for DNA condensation [pubmed|37850647]
Paralogous protein(s)
[protein|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
[wiki|Localization]
chromosome centromer [Pubmed|23475963]
Expression and Regulation
Operons
genes
[gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]-[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]
description
[Pubmed|22383849]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
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[gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]→[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]

2024-10-05 00:42:48

ghost

81

86baea39c766e9113e6cde298217d7499c849707

0AEC202CC368218B597D878B7945E0D6E2DC147A

Biological materials
Mutant
1S135 ( ''spo0J''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S135&Search=1S135 BGSC]
1S136 ( ''spo0J''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S136&Search=1S136 BGSC]
1S137 ( ''spo0J''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S137&Search=1S137 BGSC]
BKE40960 (Δ[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCATTAATCCCTTTTCCAA,  downstream forward: _UP4_TAAATGAAAAAACCATCTTT
BKK40960 (Δ[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCATTAATCCCTTTTCCAA,  downstream forward: _UP4_TAAATGAAAAAACCATCTTT
labs
[wiki|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]
References
Reviews
22934648,26706151,28075389,30863373,31254421,34220773,35638784
Original Publications
17462018,16677298,12562803,10852876,10482533,9506522,8071208,17932079,21235642,26253537,9663676,11532141,15659156,18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,14651647,19450517,19450516,14563866,23475963,21911367,24440393,24829297,24696501,25071173,25572315,25951515,26295962,28154080,28373206,28407103,29244022,16306995,29378890,30100265,30907359,31152469,16885474,31318889,34250901,34346312,35767606,35904809,36044845,37850647,38548820

EF4BD49FCD49EE97908973581478951C8FA196C0

Page visits: 8308

Time of last update: 2024-10-04 09:49:22

Author of last update: Jstuelk