hbs
168
non-specific DNA-binding protein Hbsu
locus
BSU_22790
Molecular weight
9.75 kDa
pI
9.5
function
DNA packaging, regulation of nucleoid compaction, required for the initiation of [category|SW.3.1.1|DNA replication]
product
non-specific DNA-binding protein Hbsu
essential
yes
synonyms
hbs, dbpA, hupA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0776 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,385,543 → 2,385,821
Phenotypes of a mutant
essential [Pubmed|12682299,1382620]
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The protein
Catalyzed reaction/ biological activity
binds to curved DNA without apparent sequence specificity [pubmed|9894920]
binds to abasic DNA lesions [pubmed|37250769]
condenses the bacterial chromosome [pubmed|9068655]
[protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth] endonuclease activity at AP sites [Pubmed|21954439]
plays an essential role in the initiation of [wiki|DNA replication], likely acting to promote origin melting by [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA], but also has a second [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-independent essential function [pubmed|35546541]
Protein family
bacterial histone-like protein family (with [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN], according to UniProt)
Structure
[PDB|1HUU] (Geobacillus stearothermophilus) [pubmed|2508086]
[AF|P08821]
Modification
phosphorylated on Arg-61 [Pubmed|22517742]
phosphorylation on Thr-4 [Pubmed|17218307]
can be acetylated on Lys-3, Lys-18, Lys-37, Lys-41, Lys-75, Lys-80, and Lys-86 [pubmed|27376153]
acetylation by [protein|0E6564B1B910694F28FD6EF5231E6F6A0E4BE545|yfmK] on Lys-80 inhibits DNA binding and nocleoid compaction [pubmed|30808761]
may be acetylated by [protein|07A30814F57112196051289AA309AE6DB22CAEFF|ydgE] on Lys-41 and Lys-86 [pubmed|30808761]
Effectors of protein activity
acetylation by [protein|0E6564B1B910694F28FD6EF5231E6F6A0E4BE545|yfmK] on Lys-80 inhibits DNA binding and nocleoid compaction [pubmed|30808761]
Paralogous protein(s)
[protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]
[wiki|Localization]
uniformly associated with the nucleoid in vegetative cells and forespores [Pubmed|21085634,9068655,10715001]
Expression and Regulation
Operons
genes
[gene|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]
description
[Pubmed|1902464]
regulation
induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1902464], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|1902464], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
additional information
mRNA degradation is initiated by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|28977557]
Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis [PubMed|10715001]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
labs
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
[wiki|David Dubnau],Rutgers University, Newark, USA
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:46:08
Author of last update: Jstuelk