polY2
168
translesion synthesis (TLS-) DNA polymerase Y2
locus
BSU_23710
Molecular weight
45.78 kDa
pI
9.53
function
UV-targeted mutagenesis
product
translesion synthesis (TLS-) DNA polymerase Y2
essential
no
synonyms
polY2, yqjW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0389 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,464,562 → 2,465,800
Phenotypes of a mutant
sensitive to blue light-induced DNA damage [pubmed|30054368]
increased sensitivity to Cr(VI) [pubmed|30745368]
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The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for Cr(VI)-induced mutagenesis [pubmed|30745368]
Protein family
[wiki|DNA polymerase type-Y family] (according to UniProt)
[wiki|Domains]
[wiki|UmuC domain] (aa 7-192) (according to UniProt)
Structure
[PDB|4DEZ] (from Mycobacterium smegmatis, 30% identity) [pubmed|22868761]
[AF|P54560]
Paralogous protein(s)
[protein|C87D33852F263A553CA747608E6200E4AD8344B5|polY1], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|uvrX]
Expression and Regulation
Operons
genes
[gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]-[gene|9BC3DF46C944A0FD5CAAB4513C4AF00EFC5FC8D5|yqjX]-[gene|5B8A6D282E34683726BD6C36E09A6C36E8F99795|yqjY]-[gene|9F88AC3A792F4EE8AA853849BBCF5065717B1D8B|yqjZ]-[gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]-[gene|DC1C1D4BE1A7F4E28792A2B8273AFE2C7F90E58C|yqkB]-[gene|30363923724EBD39C27967B25B486C71453190D1|yqkC]
description
[Pubmed|22383849]
regulation
induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|15469515,16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|15469515,16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
Biological materials
Mutant
MGNA-C403 (yqjW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2401 NBRP B. subtilis, Japan]
GP1505 (''polY2''::''kan''), available in [wiki|Stülke] lab [pubmed|31948638]
BKE23710 (Δ[gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACACCTCTAAAAAGA, downstream forward: _UP4_GCTAAAATAGGGGGGCATTA
BKK23710 (Δ[gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACACCTCTAAAAAGA, downstream forward: _UP4_GCTAAAATAGGGGGGCATTA
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:46:09
Author of last update: Jstuelk