ywqL
168
endonuclease V (endo V)
locus
BSU_36170
Molecular weight
26.83 kDa
pI
5.71
function
DNA excision repair
product
endonuclease V
essential
no
synonyms
ywqL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1515 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,724,420 → 3,725,136
Phenotypes of a mutant
increased mutation rates [Pubmed|22056936]
increased sensitivity of sporulating cells to the genotoxic effects of nitrous acid[Pubmed|27698084]
increased mutation rates [pubmed|30726292]
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The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate (according to UniProt)
in vitro endonuclease activity against double-stranded DNAs containing a single uracil (U), hypoxanthine (Hx), xanthine (X) or an AP site [pubmed|30726292]
catalyzes a single strand break at the second phosphodiester bond towards the 3'-end of the U and AP lesions [pubmed|30726292]
Protein family
endonuclease V family (single member, according to UniProt)
Structure
[PDB|3GA2]
[AF|P96724]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2401A4E3E5E683472C51AB2F31BC4F54F672F32A|ywqH]-[gene|04376B3166B591132CE37D1DE85B0ECFEC6902EF|ywqI]-[gene|2F9C623DDF9F93EFD46F024FDA3AF2A92FC9BF7A|ywqJ]-[gene|7ED407717C216FCB2B281A3ECFAA2ED594F021AF|ywqK]-[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
description
[Pubmed|22383849]
regulation
expressed throughout growth and staionary phase [Pubmed|22056936]
genes
[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
description
[Pubmed|9353933]
regulation
expressed throughout growth and staionary phase [Pubmed|22056936]
Biological materials
Mutant
MGNA-A566 (ywqL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/566 NBRP B. subtilis, Japan]
BKE36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
BKK36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
References
Reviews
Research papers
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Time of last update: 2024-11-29 20:46:11
Author of last update: Jstuelk