ywqL

ywqL
168

endonuclease V (endo V)

Locus
BSU_36170
Molecular weight
26.83 kDa
Isoelectric point
5.71
Protein length
238 aaSequenceBlast
Gene length
717 bpSequenceBlast
Function
DNA excision repair
Product
endonuclease V
Essential
no
Synonyms
ywqL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1515 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,724,420 → 3,725,136
Phenotypes of a mutant
increased mutation rates PubMed
increased sensitivity of sporulating cells to the genotoxic effects of nitrous acidPubMed
increased mutation rates PubMed
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The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate (according to UniProt)
in vitro endonuclease activity against double-stranded DNAs containing a single uracil (U), hypoxanthine (Hx), xanthine (X) or an AP site PubMed
catalyzes a single strand break at the second phosphodiester bond towards the 3'-end of the U and AP lesions PubMed
Protein family
endonuclease V family (single member, according to UniProt)
Structure
3GA2 (PDB)
P96724 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
ywqH-ywqI-ywqJ-ywqK-ywqL
Description
PubMed
Regulation
expressed throughout growth and staionary phase PubMed
Open in new tab

ywqH→ywqL

2025-04-08 16:28:09

ghost

81

7b0991ae05480ac928e975110362f8450bfcd9cc

255B293BB0BAC31EE5E24C2FA373EBECB756355C

Genes
ywqL
Description
PubMed
Regulation
expressed throughout growth and staionary phase PubMed
Open in new tab

ywqL

2025-04-10 13:33:21

ghost

54

fe8cd19d2b7a8898dccefa244629758f01d3a223

4BE472B104D13FED9F0546902D8EAB920CE10112

Biological materials
Mutant
MGNA-A566 (ywqL::erm), available at the NBRP B. subtilis, Japan
BKE36170 (ΔywqL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAAATACCTTCATAACGA,  downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
BKK36170 (ΔywqL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAAATACCTTCATAACGA,  downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
References
Reviews
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Research papers
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F2C6956D69FBE57933E46136436E8E80B63DF8BB

Page visits: 3293

Time of last update: 2025-04-07 21:39:53

Author of last update: Jstuelk